Xeno-free reprogramming of peripheral blood mononuclear erythroblasts
2020
Boston Children's Hospital, Boston, USA
Translating human induced pluripotent stem cell (hiPSC)–derived cells and tissues into the clinic requires streamlined and reliable production of clinical-grade hiPSCs. This article describes an entirely animal component–free procedure for the reliable derivation of stable hiPSC lines from donor peripheral blood mononuclear cells (PBMCs) using only autologous patient materials and xeno-free reagents. hiPSC lines derived using this method display expression of expected surface markers and transcription factors, loss of the reprogramming agent–derived nucleic acids, genetic stability, and the ability to robustly differentiate in vitro to multiple lineages.
Xeno-free reprogramming of peripheral blood mononuclear erythroblasts on laminin-521
Thorsten M. Schlaeger
Added on: 10-26-2022
[1] https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpsc.103
