"ID";"Original Title";"Title";"Summary";"Contact";"Citation";"URL Scientific Article";"More References";"Keywords";"Field of Research";"Method/Model";"Year of Publication";"Month of Publication";"Date of Editing"; "1680";"3D-engineered scaffolds to study microtubes and localization of epidermal growth factor receptor in patient-derived glioma cells";"3D-fabricated scaffolds for glioma research";"A major obstacle in glioma research is the lack of in vitro models that can retain the cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks are observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds, therefore, represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.";"Pim J. French, Erasmus MC Cancer Institute, Rotterdam, Netherlands, Angelo Accardo, Delft University of Technology, Delft, Netherlands";"Nastaran Barin et al. Small 2022";"https://onlinelibrary.wiley.com/doi/10.1002/smll.202204485";"";"personalised medicine, tumor, brain tumor, 3D model";"Method development, Neurology, Oncology";"Cell culture, Tissue models, Organoids, Spheroids";"2022";"10";"2022-12-14 12:08:34";