Accumulation of alpha-synuclein is a hallmark of Parkinson's disease, a highly prevalent neurodegenerative disorder. However, the dynamics of the endogenous form in the human brain are not well-understood because of the lack of samples to study and reliable methods to analyze them. In this study, a single-molecule pull-down assay combined with in vivo crosslinking is used to study alpha-synuclein in postmortem human brains. The results showed that this technique can be successfully used to study alpha-synuclein at the single-molecule level using minimum amounts of protein and that it can be used to study the oligomerization states in human cultured cells and postmortem human brain samples. Overall, the researchers propose a new powerful tool that has the potential to be used to diagnose Parkinson's disease and other diseases that involve the accumulation, oligomerization and aggregation of specific peptides.
Endogenous alpha-synuclein protein analysis from human brain tissues using single-molecule pull-down assay
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