Immortalized human cell lines are a powerful tool to model human diseases in a cost-effective manner. The human neuroblastoma cell line SH-SY5Y is a popular cell line used to study pathological features of neurodegenerative diseases, especially Parkinson's disease. However, the relevance of this model in neurodegenerative diseases has not yet been studied. Here, the SH-SY5Y cell line has been characterized using a systemic genomics approach: combining genomic, transcriptomic and proteomic data to evaluate its suitability as a model for neurodegenerative diseases. The results show that there is consistency across the different -omics analyses. Moreover, all the major pathways involved in Parkinson's disease are intact, thus it is a good candidate line to model the disease. However, the genetic integrity is not ideal for some other neurodegenerative diseases, therefore it should be taken into account as a potential limitation when using this cell line in other disorders. In this study, the SH-SY5Y cell line is characterized and validated for the first time using a system genomics approach, increasing the consistency of the model and giving useful data to the scientific community about the benefits and limitations of using this model to study neurodegenerative diseases.
Systems genomics evaluation of the SH-SY5Y neuroblastoma cell line as a model for Parkinson’s disease
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