Non Animal Testing Database

Strategy to replace animal-derived extracellular matrices

University of Konstanz, Konstanz, Germany
Many extracellular matrices (ECM) used for modern cell culture are derived from animals. An alternative approach is the recombinant production of individual matrix protein components. Self-assembling protein domains from human muscle sarcomeres, termed ZT material (ZT), have been shown to be suitable for a modular approach of generating ECMs. The study determined that ZT modified with the fibronectin 10 domain (ZTFn10) is able to substitute bovine serum-derived fibronectin as a coating for neural crest cell (NCC)-based toxicity testing. Thus, the automated version of an NCC migration assay cMINC, and possibly other bioassays, can be performed with a ZT-based ECM instead of traditional animal-derived protein coatings.
Strategy to replace animal-derived ECM by a modular and highly defined matrix
Marcel Leist, Xenia Dolde
Added on: 01-06-2021
Back to Top
English German

Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!