Many extracellular matrices (ECM) used for modern cell culture are derived from animals. An alternative approach is the recombinant production of individual matrix protein components. Self-assembling protein domains from human muscle sarcomeres, termed ZT material (ZT), have been shown to be suitable for a modular approach of generating ECMs. The study determined that ZT modified with the fibronectin 10 domain (ZTFn10) is able to substitute bovine serum-derived fibronectin as a coating for neural crest cell (NCC)-based toxicity testing. Thus, the automated version of an NCC migration assay cMINC, and possibly other bioassays, can be performed with a ZT-based ECM instead of traditional animal-derived protein coatings.
Strategy to replace animal-derived ECM by a modular and highly defined matrix
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