This study compared static 2D-cancer cell cultures to 3D- cancer cell cultures, comprising a central mass of breast cancer cells surrounded by collagen type-1 and incorporated fluid flow and pressure. The 3D-cancer model differs by its improved tumour-microenvironment. This includes an extracellular matrix as well as capillary fluid flow and interstitial fluid pressure. The cell lines MDA-MB231 and SKBR3 were employed for this study. The 3D model showed reduced sensitivity to the chemotherapeutic agent Doxorubicin and an overall more aggressive phenotype (furthermore important: downregulation of Ki67 and cadherin-11, upregulation of snail and MMP14, i.a.).
Cancer cells grown in 3D under fluid flow exhibit an aggressive phenotype and reduced responsiveness to the anti‑cancer treatment doxorubicin
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