Non Animal Testing Database
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Differences in 2D- and 3D-cancer cell cultures

2020
University of Westminster, London, United Kingdom
This study compared static 2D-cancer cell cultures to 3D- cancer cell cultures, comprising a central mass of breast cancer cells surrounded by collagen type-1 and incorporated fluid flow and pressure. The 3D-cancer model differs by its improved tumour-microenvironment. This includes an extracellular matrix as well as capillary fluid flow and interstitial fluid pressure. The cell lines MDA-MB231 and SKBR3 were employed for this study. The 3D model showed reduced sensitivity to the chemotherapeutic agent Doxorubicin and an overall more aggressive phenotype (furthermore important: downregulation of Ki67 and cadherin-11, upregulation of snail and MMP14, i.a.).
Cancer cells grown in 3D under fluid flow exhibit an aggressive phenotype and reduced responsiveness to the anti‑cancer treatment doxorubicin
Miriam V. Dwek
#396
Added on: 11-26-2020
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