Renal reabsorption in 3D kidney tissue
2019
Harvard University, Boston, USA(1)
Roche Innovation Center Basel, Basel, Switzerland(2)
Roche Innovation Center Basel, Basel, Switzerland(2)
Three-dimensional renal tissues that emulate the cellular composition, geometry, and function of native kidney tissue would enable fundamental studies of filtration and reabsorption. Here, 3D vascularized proximal tubule models were developed that are composed of adjacent conduits, lined with confluent epithelium and endothelium, embedded in a permeable extracellular matrix. They were independently addressed using a closed-loop perfusion system to investigate renal reabsorption. The developed 3D kidney tissue allowed for coculture of proximal tubule epithelium and vascular endothelium that exhibited active reabsorption via tubular–vascular exchange of solutes akin to native kidney tissue. Using this model, both albumin uptake and glucose reabsorption were quantified as a function of time. Epithelium–endothelium cross-talk was further studied by exposing proximal tubule cells to hyperglycaemic conditions and monitoring endothelial cell dysfunction. This diseased state could be rescued by administering a glucose transport inhibitor. The 3D kidney tissue provides a platform for in vitro studies of kidney function, disease modelling, and pharmacology.
Renal reabsorption in 3D vascularized proximal tubule models
Jennifer A. Lewis(1), Annie Moisan(2)
Added on: 07-29-2024
[1] https://www.pnas.org/doi/full/10.1073/pnas.1815208116
