Eye irritation test SkinEthic HCE EIT
Regulatory accepted 2015
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The SkinEthic™ Human Corneal Epithelium (HCE) Eye Irritation Test (EIT) method is an in vitro assay used for assessing eye irritation or serious eye damage. The method uses a reconstructed human cornea-like epithelium (RhCE) consisting of a multilayered epithelium prepared from immortalized human corneal epithelial cells which reproduce the multi-layer organization of the human cornea. The tissues are exposed to the test chemicals according to different possible protocols.
Validated and regulatory approved under TM2015-03 (EU) by EURL ECVAM (TSAR list), OECD Test No. 492.
SkinEthic™ HCE Eye Irritation Test
EURL ECVAM
EURL ECVAM [1]
Natalie Alépée et al. Toxicology in Vitro 2016a [2]
Natalie Alépée et al. Toxicology in Vitro 2016b [3]
OECD [4]
Added on: 07-02-2020
[1] https://tsar.jrc.ec.europa.eu/test-method/tm2015-03[2] https://www.sciencedirect.com/science/article/abs/pii/S0887233315300138?via%3Dihub[3] https://www.sciencedirect.com/science/article/abs/pii/S0887233316300303?via%3Dihub[4] https://www.oecd-ilibrary.org/environment/test-no-492-reconstructed-human-cornea-like-epithelium-rhce-test-method-for-identifying-chemicals-not-requiring-classification-and-labelling-for-eye-irritation-or-serious-eye-damage_9789264242548-en
