Non Animal Testing Database

Eye irritation test with reconstructed cornea

validierte Methode
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The EpiOcular™ Eye Irritation Test (EIT) consists of a reconstructed human cornea-like epithelium (RhCE) tissue construct, a non-keratinized multi-layered epithelium prepared from non-transformed, human-derived epidermal keratinocytes which show a cornea-like structure analogous to that found in vivo. The test chemical is applied topically to a minimum of two RhCE tissue constructs. Following the exposure and post-treatment incubation periods, tissue viability is assessed by MTT assay. Validated and regulatory accepted under TM2008-10 (EU) by EURL ECVAM (TSAR list), OECD Test No. 492.
EpiOcular™ Human Cell Construct EIT
Added on: 07-02-2020
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