Eye irritation test with reconstructed cornea
Regulatory accepted 2008
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The EpiOcular™ Eye Irritation Test (EIT) consists of a reconstructed human cornea-like epithelium (RhCE) tissue construct, a non-keratinized multi-layered epithelium prepared from non-transformed, human-derived epidermal keratinocytes which show a cornea-like structure analogous to that found in vivo. The test chemical is applied topically to a minimum of two RhCE tissue constructs. Following the exposure and post-treatment incubation periods, tissue viability is assessed by MTT assay. Validated and regulatory accepted under TM2008-10 (EU) by EURL ECVAM (TSAR list), OECD Test No. 492.
EpiOcular™ Human Cell Construct EIT
EURL ECVAM
Added on: 07-02-2020
[1] https://tsar.jrc.ec.europa.eu/test-method/tm2008-10[2] https://www.oecd-ilibrary.org/environment/test-no-492-reconstructed-human-cornea-like-epithelium-rhce-test-method-for-identifying-chemicals-not-requiring-classification-and-labelling-for-eye-irritation-or-serious-eye-damage_9789264242548-en[3] https://journals.sagepub.com/doi/abs/10.1177/026119291103900409
