Non Animal Testing Database

Pyrogen test using human blood

validierte Methode
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
The in vitro pyrogen test using human cryopreserved whole blood/IL-1 Beta (Cryo-WBT-IL1) is a monocyte activation test (MAT) used to detect or quantify substances that activate human monocytes or monocytic cells to release pro-inflammatory cytokines, for example, tumour necrosis factor-alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). These cytokines have a role in fever pathogenesis. Consequently, the MAT can detect the presence of pyrogens in the test sample. The MAT is suitable, after a product-specific validation, as a replacement for the rabbit pyrogen test or the bacterial endotoxin test (Limulus amebocyte lysate assay). Validated and regulatory approved under TM2002-02 (EU) by EURL ECVAM (TSAR list).
In vitro pyrogen test using human cryopreserved whole blood/IL-1 beta (Cryo-WBT-IL1)
Added on: 07-02-2020
Back to Top
English German

Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!