Non Animal Testing Database
EnglischDeutsch

HepaRG™ as a test for hepatotoxicity

validierte Methode
2009
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy
HepaRG™ is a human-based liver metabolism test system. It is based on the activation of three specific liver enzymes upon HepaRG™ cells exposure to liver-damaging substances. First, the liver cells are exposed to the test chemical. Next, enzyme substrates are added to the cells and the enzyme activity, as well as the metabolite formation of the substrates, is measured by liquid chromatography/mass spectrometry (LC/MS) to determine the liver toxic potential of the test chemical. The method has been validated under TM2009-14 (EU) and regulatory acceptance is ongoing.
Cytochrome P450 (CYP) enzyme induction in vitro method using cryopreserved differentiated human HepaRG™ cells
EURL ECVAM
#197
Added on: 07-02-2020
Back to Top
English German

Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!