Non Animal Testing Database

Short-time exposure in vitro method for assessment of eye damage

Validated Method
European Union Reference Laboratory for Alternatives to Animal Testing, Ispra, Italy(1)
OECD Guidelines for the Testing of Chemicals, Paris, France(2)
The Short Time Exposure (STE) test method is an in vitro method for chemicals inducing eye damage or irritation. The SIRC (Statens Seruminstitut Rabbit Cornea cells) cell line, available via well-qualified cell banks, is used. After the exposure of the cells to the test chemical, the cytotoxicity is quantitatively measured as the relative viability of SIRC cells using the MTT (Thiazolyl Blue Tetrazolium Bromide) assay. Decreased cell viability is used to predict potential adverse effects leading to ocular damage. The obtained cell viability is compared to the control and used to estimate the potential eye hazard of the test chemical. Validated and regulatory accepted under OECD test No. 491.
Test No. 491. Short time exposure in vitro test method for identifying i) Chemicals inducing serious eye damage and ii) Chemicals not requiring classification for eye irritation or serious eye damage
Added on: 07-11-2023
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