Non Animal Testing Database

Investigation of autophagy processes in lung cells

University of Southern California, Los Angeles, USA
Autophagy, a homeostatic mechanism, is crucial in maintaining normal cellular function. Although dysregulation of autophagic processes is recognized in certain diseases, it is unknown how maintenance of cellular homeostasis might be affected by the kinetics of autophagic activity in response to various stimuli. In this study, the researchers assessed those kinetics in lung adenocarcinoma (A549) cells in response to exposure to nanoparticles (NP) and/or Rapamycin. Since NP are known to induce autophagy, the authors wished to determine if this phenomenon could be a driver of the harmful effects seen in lung tissue exposed to air pollution. Autophagic activity was assessed in live single cells using confocal laser scanning microscopy. Autophagic activity over time was determined during exposure of A549 cells to single or double agents known to stimulate autophagy. Autophagic activity increased in all experimental modalities. These results are consistent with the hypothesis that environmental stressors might exert their harmful effects, at least in part, by limiting available autophagic response to additional stimulation, thereby making nanoparticle-exposed cells more susceptible to secondary injury due to autophagic overload.
Kinetics of autophagic activity in nanoparticle-exposed lung adenocarcinoma (A549) cells
Arnold Sipos
Added on: 06-05-2023
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