Changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate models have limited the understanding of how such substances impact human brain metabolism. Here a shotgun mass spectrometry was used to explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids. Out of the 6,728 identified proteins, 934 were found differentially expressed in 5-MeO-DMT-treated cerebral organoids. In silico analysis reinforced previously reported anti-inflammatory actions of 5-MeO-DMT and revealed modulatory effects on proteins associated with long-term potentiation, the formation of dendritic spines, including those involved in cellular protrusion formation, microtubule dynamics, and cytoskeletal reorganization. The presented data offer the first insight about molecular alterations caused by 5-MeO-DMT in human cerebral organoids.
Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
Stevens K. Rehen
Added on: 05-09-2023