Non Animal Testing Database

Autophagy of human macrophages infected with Mycobacterium tuberculosis

The Francis Crick Institute, London, United Kingdom
Autophagy is a cellular innate-immune defence mechanism against intracellular microorganisms, including Mycobacterium tuberculosis (Mtb). How canonical and non-canonical autophagy function to control Mtb infection in phagosomes and the cytosol remains unresolved. Macrophages are the main host cell in humans for Mtb. Here, the researchers studied the contributions of canonical and non-canonical autophagy in the genetically tractable human induced pluripotent stem cell-derived macrophages (iPSDM), using a set of Mtb mutants. Using Mtb reporters and quantitative imaging, they identified a role for the ATG14 protein in regulating the fusion of phagosomes containing Mtb with lysosomes, thereby enabling intracellular bacteria restriction. The study shows that ATG7 and ATG14 are both required for restricting Mtb replication in human macrophages.
ATG7 and ATG14 restrict cytosolic and phagosomal Mycobacterium tuberculosis replication in human macrophages
Maximiliano G. Gutierrez
Added on: 05-02-2023
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