Non Animal Testing Database

Optogenetic human pancreatic cell line

IMC University of Applied Sciences, Krems, Austria
Augmented Toll-like receptor 4 (TLR4) expression was found in nearly 70% of patients with pancreatic adenocarcinoma, which is correlated with increased tumorigenesis and progression. In this study, the researchers engineered a new light-oxygen-voltage-sensing (LOV) domain-based optogenetic cell line (opto-TLR4 PANC-1) that enables the triggering of certain signalling cascades upon blue light-sensitive homodimerisation of the TLR4-LOV fusion protein. Using this cell line, the researchers performed different phenotypic cell-based assays with 2D and 3D cultures, with the aim of controlling cellular activity with spatial and temporal precision. Light exposure enhanced cell attachment, the formation, and extension of invadopodia, and cell migration in 3D spheroid cultures, but no significant changes in proliferation or viability could be detected. The authors conclude that the opto-TLR4 PANC-1 cell line is an ideal tool for investigating the underlying molecular mechanisms of TLR4, thereby providing strategies for new therapeutic options.
Light-inducible spatio-temporal control of TLR4 and NF-κB-Gluc reporter in human pancreatic cell line
Christoph Wiesner
Added on: 12-16-2022
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