Drug-induced phenotypic landscape of colorectal cancer organoids
2022
German Cancer Research Center (DKFZ), Heidelberg, Germany(1)
University Medical Center Mannheim, Mannheim, Germany(2)
University Medical Center Mannheim, Mannheim, Germany(2)
Here, a large-scale image-based phenotyping study of patient-derived cancer organoids was reported to understand underlying factors governing organoid morphology. Colorectal cancer organoids from 11 patients were treated with more than 500 experimental and clinically used small molecules at different concentrations. The morphological heterogeneity of patient-derived organoids and their response to compound perturbations from more than 3,700,000 confocal microscopy images were mapped. The authors found that the resulting landscape of organoid phenotypes was mainly driven by differences in organoid size, viability and cystic vs. solid organoid architecture. Using multi-omics factor analysis for integrating organoid morphology, size, gene expression, somatic mutations and drug activity, biological programs underlying these phenotypes and small molecules that modulate them were identified.
The drug-induced phenotypic landscape of colorectal cancer organoids
Michael Boutros(1), Matthias P. Ebert(2)
Added on: 12-15-2022
[1] https://www.nature.com/articles/s41467-022-30722-9
