Non Animal Testing Database

Drug-induced phenotypic landscape of colorectal cancer organoids

German Cancer Research Center (DKFZ), Heidelberg, Germany(1)
University Medical Center Mannheim, Mannheim, Germany(2)
Here, a large-scale image-based phenotyping study of patient-derived cancer organoids was reported to understand underlying factors governing organoid morphology. Colorectal cancer organoids from 11 patients were treated with more than 500 experimental and clinically used small molecules at different concentrations. The morphological heterogeneity of patient-derived organoids and their response to compound perturbations from more than 3,700,000 confocal microscopy images were mapped. The authors found that the resulting landscape of organoid phenotypes was mainly driven by differences in organoid size, viability and cystic vs. solid organoid architecture. Using multi-omics factor analysis for integrating organoid morphology, size, gene expression, somatic mutations and drug activity, biological programs underlying these phenotypes and small molecules that modulate them were identified.
The drug-induced phenotypic landscape of colorectal cancer organoids
Michael Boutros(1), Matthias P. Ebert(2)
Added on: 12-15-2022
Back to Top
English German

Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!