Development of immunologically active drugs with co-cultures
HOT Screen GmbH, Reutlingen, Germany
For the development of immunologically active drugs and in the context of immunotoxicology, the cellular dialogue between the cells of the immune system and those of the tissue is of great importance. For this purpose, HOT Screen offers complex cell culture models, each containing two compartments, one for the immune cells and one for the cells from different tissues. These cells can be incubated with the test substances under different conditions. The effects of the substances on the cellular interactions between the two compartments are detected by measuring those mediators or cellular products that can be considered most specific for each of the cell types in the culture. By choosing different activation signals and stimulation times, it is possible to create a variety of culture conditions, e.g., to simulate pathogenetically relevant situations. To map the absorbability of immunologically active drugs by the intestinal epithelium, a co-culture system is provided consisting of cells of the human immune system and a terminally differentiated, fully functional human intestinal epithelium (derived from CaCo-2 cells). This complex culture system allows a detailed study not only of the bioavailability of test compounds but also of their influence on the regulatory interactions between these two tissues in situations of intestinal immune activation. Currently available "HOT-Co" test systems exist, each in combination with whole blood, for intestine (with intestinal epithelium), joint (with synovial fibroblasts), skin (3D epidermis), lung I and II (with alveolar cells or bronchial cells), vessels (with endothelial cells) or diabetes (with fat cells).
Added on: 12-15-2022