Non Animal Testing Database

Co-culture model of inflammatory bowel disease

November 2022
Saarland University, Saarbruecken, Germany
This study shows the development of an in-vitro assay for the screening of inflammatory bowel disease (IBD)-related drugs. Therefore, a co-culture of human colorectal cells (Caco-2) with monocyte-derived macrophages was developed, addressing the intestinal barrier and the immune response in IBD. The model was exposed to known drugs, and the drug efficacy was analyzed after lipopolysaccharide (LPS)-induced inflammation of the co-culture and drug exposure. Read-out was performed by transepithelial electrical resistance (TEER) measurement and monitoring of cytokine release. The developed in vitro assay offers an easy and scalable method for IBD drug screening.
Inflammatory bowel disease addressed by Caco‑2 and monocyte‑derived macrophages: an opportunity for an in vitro drug screening assay
Marius Hittinger
Added on: 12-12-2022
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