Non Animal Testing Database

Human 3D cartilage model of osteoarthritis

Leiden University Medical Centre, Leiden, Netherlands
In this study, a human in vitro 3D cartilage model was developed that enables the exploration of the responses of osteoarthritis risk genes to mechanical stress. Human primary chondrocytes were isolated from the joints of patients undergoing total joint replacement and used to form 3D cartilage pellets representing physiological cartilage. These pellets were exposed to dynamic mechanical stress. Gene expression was quantified and histological analysis and immunohistochemistry were performed. Mechanical stress-induced cartilage damage and effects on the extracellular matrix (ECM) and expression of genes were monitored, including osteoarthritis risk genes. The model could be a suitable platform for high throughput screening of potential drugs targeting involved genes.
A human in vitro 3D neo-cartilage model to explore the response of OA risk genes to hyper-physiological mechanical stress
Ingrid Meulenbelt
Added on: 12-12-2022
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