3D bioprinted cartilage tissue of iPS cells in a nanocellulose/alginate bioink
2017
University of Gothenburg, Gothenburg, Sweden
Cartilage lesions can develop into secondary osteoarthritis and cause severe clinical problems in numerous patients. To treat such lesions, this study demonstrated that human induced pluripotent stem cells (iPSCs) can be processed into 3D bioprints of cartilage imitations using a composite of nanofibrillated cellulose (NFC) and irradiated human chondrocytes. Two bioprints were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). In the case of NFC/HA, low proliferation and phenotypic changes away from pluripotency were observed. However, in the 3D-bioprinted NFC/A constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilage tissue with collagen type II expression and without tumorigenic Oct4 expression was observed in the 3D-bioprinted NFC/A constructs. In addition, 2-photon fluorescence microscopy showed a significant increase in cell number within the cartilage tissue, indicating the importance of high cell densities for good survival after printing. The results indicate that NFC/A bioprinting ink is suitable for bioprinting iPSCs to support cartilage production in co-cultures with irradiated chondrocytes.
Cartilage tissue engineering by the 3D bioprinting of iPS cells in a nanocellulose/alginate bioink
Stina Simonsson
Added on: 11-14-2022
[1] https://www.nature.com/articles/s41598-017-00690-y#Sec4
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