In this study, the principle of electrochemical Localised Oxygen Scavenging (eLOS) was studied to apply acute hypoxia in in-vitro cell models. Therefore, the oxygen gradient was investigated next to a polarized electrode, and a design was developed to induce the spatiotemporal hypoxia via electrochemical oxygen scavenging.
The system was evaluated using human neural progenitor cells, to show the spatiotemporal gradient of the hypoxic response. The platform was further integrated into a microchannel device to study a human cortical infarct model with pluripotent stem cell (PSC)-derived human neurons. It was demonstrated that eLOS can improve control of hypoxia in timescales of acute and chronic hypoxia.
The versatile system can be further adapted for studying tumor microenvironment, myocardial infarction and other ischaemic systems in-vitro.
Electrochemically induced in vitro focal hypoxia in human neurons
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