Non Animal Testing Database
EnglischDeutsch

Skin permeation in ex vivo human skin visualized using mass spectrometry

October 2021
Chalmers University of Technology, Gothenburg, Sweden
Skin permeation and distribution of three of the most common skin sensitizers were investigated using a previously developed animal-free exposure method combined with imaging mass spectrometry. Nickel, cobalt, and chromium (III) salts were dissolved in a buffer and exposed to human skin ex vivo, to be analysed using time of flight secondary ion mass spectrometry (ToF-SIMS). The findings demonstrate that metal haptens mainly accumulated in the stratum corneum, however, all three metal sensitizers could also be detected in the epidermis. This method provided permeation profiles in human skin for known sensitizers, on a level of detail that is not possible to achieve by other means. The findings show that the permeation profiles are different, despite these sensitizers being all metal ions and common causes of contact allergy. Studying skin uptake by only considering penetration through the skin might therefore not give accurate results.
Skin permeation of nickel, cobalt and chromium salts in ex vivo human skin, visualized using mass spectrometry imaging
Per Malmberg
#1586
Added on: 10-26-2022
Back to Top
English German
Lush Prize Winner 2022Lush Prize Winner 2022
Winner of the Animal Protection Prize of Lower Saxony 2022Winner of the Animal Protection Prize of Lower Saxony 2022


Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!