Non Animal Testing Database

Generation of motor neurons for botulinum neurotoxin activity assays

University of Veterinary Medicine Hannover, Hannover, Germany
Within this study, three protocols for the differentiation of human induced pluripotent stem cells were compared with regard to the efficient generation of motor neurons suitable for botulinum neurotoxins (BoNT) potency estimation with cell-based assays. The differentiation protocols were analyzed regarding their motor neuron yield and the neurons' capacity to generate corresponding receptors and substrates for all BoNT serotypes. The protocol resulting in differentiated cells most sensitive to BoNTs was identified. The resulting cells are more sensitive to BoNT/A1 than the commonly used neuroblastoma cell line SiMa. It was demonstrated that human motor neurons can be the foundation of sensitive and physiologically relevant in-vitro assays for the potency estimation of botulinum neurotoxins.
Analysis of motor neurons differentiated from human induced pluripotent stem cells for the use in cell-based botulinum neurotoxin activity assays
Bettina Seeger
Added on: 08-08-2022
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