This in vitro procedure is suitable for the identification of chemicals causing eye irritation or serious eye damage. Reconstructed human cornea-like epithelium from human immortalized corneal epithelial cells is used for this assay. The SkinEthic™ HCE TTT (Human Corneal Epithelium Time-to-Toxicity) tissue construct consists of at least 4 viable cell layers. There are protocols for liquids and for solids. Cytotoxicity is measured by the reduction of a dye, while the viability of the eye-like tissue is determined after two or three exposure times with the test chemical compared to a negative control (%viability). In contrast to TG 492, cytotoxicity is also measured at different time points. Validated and regulatory accepted under OECD test No. 492B.
Reconstructed human cornea-like epithelium (RHCE) test method for eye hazard identification
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