Electrochemical impedance spectroscopy (EIS) is a non-invasive, reliable and efficient method to analyze the barrier integrity of in vitro tissue models, so far under static conditions. Here, we present a bioreactor system that allows automated, continuous, and noninvasive monitoring of cellular barrier integrity during dynamic culture. Polydimethylsiloxane (PDMS) casting and 3D printing were used to fabricate the bioreactors. To test the monitored bioreactor system, in vitro models of the blood-brain barrier (BBB) were cultured from human pluripotent stem cells (hiPSC) for up to 7 days. The transendothelial/epithelial resistance (TEER) level was found to decrease over time, which was also true for the static control culture. This versatile system offers the possibility to be used for various dynamic tissue cultures that require a non-invasive monitoring system for barrier integrity.
Online measurement system for dynamic flow bioreactors to study barrier integrity of hiPSC-based blood–brain barrier in vitro models
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