Here the researchers present a three-dimensional (3D) intestinal tissue model that can be used to study intestinal infections in humans at a level of detail that cannot be achieved with conventional two-dimensional monocultures. The model includes epithelial and endothelial layers, a primary intestinal collagen scaffold, and immune cells. Following Salmonella infection, the model mimics human gastroenteritis by confining the pathogen to the epithelial compartment. Application of dual transcriptome sequencing to the Salmonella-infected model revealed communication among epithelial, endothelial, monocyte, and natural killer cells and with the pathogen. The results suggest that Salmonella use its type III secretion systems to manipulate STAT3-dependent inflammatory responses locally in the epithelium without concomitant changes in the endothelial compartment. The approach promises to uncover additional human-specific infection strategies for Salmonella and other pathogens.
An advanced human intestinal coculture model Reveals compartmentalized host and pathogen strategies during salmonella infection
Leon N. Schulte(1), Marco Metzger(2), Jörg Vogel(3)
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