Here, a new in vitro model for epidermal wound healing (EWH) based on the reconstructed human epidermis (RHE) grown on a cell culture membrane was described. After tissue maturation, the RHE models were wounded with a biopsy punch. In addition to standard analysis techniques such as observation of progressive wound closure in phase contrast and laser scanning microscopy and histological staining of cross-sections, impedance spectroscopy was introduced as a sensitive tool to quantitatively measure wound closure and epidermal barrier restoration. Using this method, it is now possible to non-destructively monitor the development of the skin barrier for each model over a culture period of 14 days post wounding.
Reepithelialization in focus: Non-invasive monitoring of epidermal wound healing in vitro
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