3D test system for investigation of reepithelialization of reconstructed epidermis
2019
Fraunhofer-Institute for Silicate Research ISC, Wuerzburg, Germany
Here, a new in vitro model for epidermal wound healing (EWH) based on the reconstructed human epidermis (RHE) grown on a cell culture membrane was described. After tissue maturation, the RHE models were wounded with a biopsy punch. In addition to standard analysis techniques such as observation of progressive wound closure in phase contrast and laser scanning microscopy and histological staining of cross-sections, impedance spectroscopy was introduced as a sensitive tool to quantitatively measure wound closure and epidermal barrier restoration. Using this method, it is now possible to non-destructively monitor the development of the skin barrier for each model over a culture period of 14 days post wounding.
Reepithelialization in focus: Non-invasive monitoring of epidermal wound healing in vitro
Lisa Kiesewetter
Added on: 07-07-2022
[1] https://www.sciencedirect.com/science/article/abs/pii/S0956566319306347?via%3Dihub
