Non Animal Testing Database

3D alveolar model for assessment of fibrotic potential of aerosols

University of Fribourg, Fribourg, Switzerland
In this study, a three-dimensional alveolar model (EpiAlveolar) consisting of human primary alveolar epithelial cells, fibroblasts, and endothelial cells, with or without macrophages was developed for predicting long-term responses to aerosols. The model was shown to recapitulate relevant lung phenotypes and functions and was stable at the air-liquid interface for at least 3 weeks. To mimic a realistic human exposure scenario, repeated, long-term exposures were conducted at low concentrations. Proinflammatory and profibrotic responses based on the adverse outcome pathway concept for lung fibrosis were assessed upon repeated subchronic exposures to two types of carbon nanotubes and silica quartz particles. Specific key events leading to lung fibrosis, such as barrier integrity and release of proinflammatory and profibrotic markers, confirmed the responsiveness of the model.
Use of EpiAlveolar lung model to predict fibrotic potential of multiwalled carbon nanotubes
Barbara Rothen-Rutishauser
Added on: 07-06-2022
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