In this study, the differentiation protocol for human induced pluripotent stem cells (iPSC) towards small intestinal epithelial cells is optimized. Therefore, maturation of 2D-cultured iPCSs toward small intestinal epithelial cells was compared for cultivation with or without serum, and either on Geltrex or on a recombinant laminin-based substrate. Differentiation status was characterized by qPCR, immunofluorescence imaging, and functional assays. Differentiation toward definitive endoderm was shown to be more efficient without serum. Both collagen- and recombinant laminin-based coatings supported the differentiation of small intestinal epithelial cells from iPSC equally well. The resulting intestinal epithelium could be used to evaluate transcellular transport and to investigate drug adsorption.
Toward xeno-free differentiation of human induced pluripotent stem cell-derived small intestinal epithelial cells
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