An air-liquid interface (ALI) exposure system for reproducible exposure of cells to gases was developed in this study. Optimization of the exposure conditions (temperature, humidity and airflow) resulted in stable and reproducible delivery and reliable monitoring of the test atmospheres for human lung cancer (A549) cells at the ALI. Toxicologically-relevant biological responses were assessed during exposure of cells to air or ozone. The described optimization process is critical to ensure that toxicity can be attributed to the action of the test agent, rather than to suboptimal exposure conditions. This facilitates the development of advanced in vitro-based approaches for inhalation toxicology.
Establishing an air-liquid interface exposure system for exposure of lung cells to gases
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