In this study, a human pluripotent stem cell (hPSC)-based approach to generate organoids which interact with vascular cells in a spatially defined manner is described. The spatial interaction between organoid and vasculature is enabled by the use of a 3D printed microfluidic chip which allows for a sequential and developmentally matched co-culture system. It is demonstrated that on-chip hPSC-derived pericytes and endothelial cells sprout and self-assemble into organized vascular networks. Cerebral organoids are used as a model system to explore interactions with this de novo-generated vasculature. Upon co-development, vascular cells physically interact with the cerebral organoid and form an integrated neurovascular organoid. The 3D printing-based platform is designed to be compatible with any organoid system and is an easy and cost-effective way to vascularize organoids.
Engineering neurovascular organoids with 3D printed microfluidic chips
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