Non Animal Testing Database
EnglischDeutsch

Bronchial epithelial model for toxicity testing

2020
National Institute for Public Health and the Environment, Bilthoven, Netherlands
The development of in vitro toxicity testing of airborne particles involves generating air-liquid interface exposure systems that are very demanding for cell culture models. In fact, cell viability problems are common in different cell lines and this limits the exposure times. Thus, less-realistic conditions of exposure have to be applied when performing the tests. Here, a human bronchial epithelial cell line was used to develop a model based on air-liquid interface conditions for toxicity tests. The results showed that the model could be maintained for several weeks retaining a physiological phenotype. Moreover, it was possible to expose the model to continuous airflow to apply more realistic experimental conditions to airborne particles. Overall, the researchers develop a model that can be used to investigate the effect on bronchial epithelial cells of long-term exposure to toxic airborne particles in a realistic microenvironment.
An air-liquid interface bronchial epithelial model for realistic, repeated inhalation exposure to airborne particles for toxicity testing
Hedwig M Braakhuis
#1291
Added on: 11-30-2021
Back to Top
English German

Warning: Internet Explorer

The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.

Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!