A better understanding of neurodevelopment is necessary to properly comprehend brain physiology in different conditions. Stem cell technologies advances in recent years allow the generation of human models through the recapitulation of human development in vitro. However, there are still certain limitations and a proper evaluation of the protocols should be done by comparing the in vitro process to their in vivo counterparts. Here, human samples of the whole ganglionic eminence and adult striatum are processed and analyzed by quantitative high-throughput gene expression analysis to elucidate the gene expression patterns that drive striatum development. The results showed that the relative expression of specific genes between brain areas is a key factor in their proper development. Afterwards, these expression profiles were used to characterize the differentiation of human pluripotent stem cells through whole ganglionic eminence identity towards adult striatum-like cells. Overall, the researchers establish a transcriptomic profile to evaluate stem cell-derived tools for in vitro modelling or cell therapy strategies.
Quantitative high-throughput gene expression profiling of human striatal development to screen stem cell–derived medium spiny neurons
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