Electroporation is a common technique usually applied to suspension or monolayer cultures. However, 3D systems better reproduce the complex in vivo conditions and cell-matrix interactions. Recent studies have applied electroporation to 3D models replicating in vivo conditions, but still with important limitations. Here, a 3D macroscopic myxoid matrix scaffold to culture human breast epithelial cancer cells was developed to mimic myxoid stroma tumours and replicate the cell-cell and cell-matrix interactions existing in vivo. The results showed that the newly developed platform induced cell proliferation and allows the construction of cell-produced fibrous structures that correlate positively with culture time. Moreover, this system allowed to efficiently electroporate cells cultured on the scaffold. Overall, the researchers propose a new culture system that reproduces key features of in vivo myxoid stroma tumours and can be used to investigate the applicability of electrochemotherapy in cancer.
Cell-seeded 3D scaffolds as in vitro models for electroporation
The IE from MS no longer understands current scripting languages, the latest main version (version 11) is from 2013 and has not been further developed since 2015.
Our recommendation: Use only the latest versions of modern browsers, for example Google Chrome, Mozilla Firefox or Microsofrt Edge, because only this guarantees you sufficient protection against infections and the correct display of websites!