Reduction of cell stress with mirtazapine in a cell model of depression
2022
University of Porto, Porto, Portugal
Depression is associated with a high relapse rate, which makes its treatment a challenge. Therefore, intensive research into the disease and possible treatments is needed. In this study, cell viability assays (MTT and neutral red assays) and alkaline comet assays were used to test the induction of stress in human SH-SY5Y cells by the application of hydrocortisone and hydrogen peroxide and the reversal or attenuation of this stress by the application of mirtazapine to the cells. The results showed that hydrogen peroxide, but not hydrocortisone, can induce cellular stress, as evidenced by DNA damage and a global reduction in cellular viability, which could be alleviated by the antidepressant mirtazapine.
The establishment of a cellular model of depression by stress induction is important in order to investigate new possibilities for the treatment of this disease with the aid of cell cultures.
Cell model of depression: reduction of cell stress with mirtazapine
Nuno Vale
Added on: 03-27-2024
[1] https://www.mdpi.com/1422-0067/23/9/4942
