Novel markers for human cell line activation test
2021
Singapore Immunology Network, Singapore, Singapore
The human cell line activation test (h-CLAT) is an OECD-approved (Test No. 442E) assay to identify novel skin sensitizers. h-CLAT simulates dendritic cell activation in the skin sensitization pathway and is based on the measurement of CD54 and CD86 overexpression on monocytic, leukaemic THP-1 cells. However, the current h-CLAT markers show inconsistent results with moderate and weak sensitizers. Moreover, these markers have accessory roles in cell adhesion and signalling rather than a direct role in cellular inflammation. Therefore, the researchers have explored other inflammation-related markers using peripheral blood mononuclear cells (PBMCs) and THP-1 cells. They identified five novel markers that may supplement the current repertoire of h-CLAT markers.
Preliminary discovery of novel markers for human cell line activation test (h-CLAT)
Ee Chee Ren
Added on: 12-02-2022
[1] https://www.sciencedirect.com/science/article/abs/pii/S0887233321000795?via%3Dihub
