Microfluidic coculture of liver organoids and T-cells
2022
California Pacific Medical Center & Research Institute, San Francisco, USA(1)
The Gladstone Institutes, San Francisco, USA(2)
The Gladstone Institutes, San Francisco, USA(2)
This study describes a microfluidic chip in which 3D human liver organoids and human T-cells are cocultured, thereby allowing for the observation of T-cell invasion and morphological changes in the liver organoids. The microfluidic coculture system supports the targeted killing of liver organoids when pulsed with a peptide derived from the hepatitis C virus (HCV) in the presence of patient-derived T-cells specific for the peptide. This demonstrates the potential of the coculture system to molecularly study adaptive immune responses to HCV in an in vitro setting using primary human cells. Additionally, the microfluidic chip allows the incorporation of additional relevant immune or liver cell types to understand their role in HCV infection. The platform can be used to gain insight into HCV immunobiology, potentially accelerating the already long path towards an effective vaccine.
Modelling T-cell immunity against hepatitis C virus with liver organoids in a microfluidic coculture system
Stewart Cooper(1), Melanie Ott(2), Todd C. McDevitt(2)
Added on: 03-31-2022
[1] https://royalsocietypublishing.org/doi/10.1098/rsob.210320
