Long-term cultivation of lung slices
2022
Hannover Medical School, Hanover, Germany
Human precision-cut lung slices (PCLS) have proven to be useful for numerous toxicologic, pharmacologic, and immunologic studies. They are widely used to study the pathophysiology, mechanisms, and therapy of different lung diseases because PCLS highly resemble the original tissue. Although a cultivation period of < 1 week is sufficient for most studies, modelling complex disease mechanisms and investigating the effects of long-term exposure to certain substances require cultivation periods that are much longer. To investigate the effects of long-term cultivation on PCLS, more than 1500 human PCLS from 16 different donors were cultivated under standardized, serum-free conditions for up to 28 days. The viability, tissue integrity, and transcriptome were assessed. Overall, this study provides a comprehensive overview of histomorphologic and pathologic changes during the long-term cultivation of PCLS. Moreover, the implications of these changes on various applications of PCLS are discussed.
The challenge of long-term cultivation of human precision-cut lung slices
Eike B. Preuß
Added on: 03-16-2022
[1] https://ajp.amjpathol.org/article/S0002-9440(21)00481-8/fulltext
